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SRX9427743: GSM4879091: DMZ.13_rep2; Xenopus laevis; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 40.1M spots, 12.1G bases, 4.6Gb downloads

Submitted by: NCBI (GEO)
Study: A temporally resolved transcriptome for developing “Keller” explants of the Xenopus laevis dorsal marginal zone
show Abstracthide Abstract
Explanted tissues from vertebrate embryos reliably develop in culture and have provided essential paradigms for understanding embryogenesis, from early embryological investigations of induction, to the extensive study of Xenopus animal caps, to the current studies of mammalian gastruloids. Cultured explants of the Xenopus dorsal marginal zone (“Keller” explants) serve as a central paradigm for studies of convergent extension cell movements, yet we know little about the global patterns of gene expression in these explants. In an effort to more thoroughly develop this important model system, we provide here a time-resolved bulk transcriptome for developing Keller explants. Overall design: RNA-Seq analysis of a timecourse of dorsal marginal zone explants from Xenopus embryos from stage 11 to stage 18.
Sample: DMZ.13_rep2
SAMN16655519 • SRS7642506 • All experiments • All runs
Organism: Xenopus laevis
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: When sibling embryos became stage 11, 11.5, 12, 12.5, 13, 14, 15, 16, 17, and 18, five or six DMZs were collected and dissolved in TRIzol Reagent for each time point. The time interval between the stages was approximately 30 minutes to an hour at room temperature. 20 animal caps and five VMZs were excised from stage 8 and 10.5, respectively. Animal caps and VMZs were dissolved in TRIzol when sibling embryos were at stage 11. Standard illumina RNA-Seq library preparation at a core facility
Experiment attributes:
GEO Accession: GSM4879091
Links:
Runs: 1 run, 40.1M spots, 12.1G bases, 4.6Gb
Run# of Spots# of BasesSizePublished
SRR1297555040,102,48712.1G4.6Gb2020-11-04

ID:
12318919

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